The 2000 Calder Cup Playoffs of the American Hockey League began on April 11, 2000. The sixteen teams that qualified, eight from each conference, played best-of-5 series for division semifinals and best-of-7 series for division finals and conference finals. The conference champions played a best-of-7 series for the Calder Cup. The Calder Cup Final ended on June 4, 2000 with the Hartford Wolf Pack defeating the Rochester Americans four games to two to win the first Calder Cup in team history.
After the 1999-2000 AHL regular season, 16 teams qualified for the playoffs. The top four teams from each division qualified for the playoffs. However, due to the uneven number of teams in the Eastern Conference, it was possible for the fifth-placed team in the New England Division to crossover to the Atlantic Division. This could only happen if the fifth-placed team in the New England Division earned more points than the fourth-placed team in the Atlantic Division. In this case, the fifth-placed team from the New England Division would play in place of the fourth-placed team from the Atlantic Division in that part of the playoff bracket. The Hartford Wolf Pack were the Eastern Conference regular season champions as well as the Macgregor Kilpatrick Trophy winners with the best overall regular season record. The Rochester Americans were the Western Conference regular season champions.
In each round the team that earned more points during the regular season receives home ice advantage, meaning they receive the "extra" game on home-ice if the series reaches the maximum number of games. There is no set series format due to arena scheduling conflicts and travel considerations.